Article Text

PDF

Other themes
SP6-28 Effect of umbilical cord blood acid-base status and gas values on the yield of mononuclear cells and cd34 cells
  1. S S Ebina1,
  2. A Omori1,
  3. A Tarakida2,
  4. T Ogasawara2,
  5. M Manabe2,
  6. S I Katagiri2,
  7. I Kashiwakura1
  1. 1Hirosaki University Graduate School of Health Sciences, Hirosak, Aomori, Japan
  2. 2Hirosaki National Hospital, Hirosak, Aomori, Japan

Abstract

Purpose To investigate the influence of umbilical cord blood (CB) acid-base status and gas values on the yield of mononuclear cells and CD34 cells, pH, pCO2, pO2, HCO3 and base excess were measured in arterial CB samples obtained from normal full-term deliveries. The relationship of these values with the yield of mononuclear cells and CD34 cells detected in venous CB was analysed.

Methods A total of 145 CB units were collected from full-term vaginal deliveries at a single hospital. Immediately after delivery, a segment of the umbilical cord was double clamped, and arterial CB was analysed to determine the acid-base status and gases. Venous CB was collected into a sterile collection bag and processed for cell separation within 24 h collection. The relationship between umbilical arterial acid-base status, each gas value, and the yield of mononuclear cells and CD34 cells was analysed.

Results Statistically significant correlations were observed between the net weight of CB and the total mononuclear and CD34 cell counts. In addition, there was a negative correlation between the mononuclear cell counts and pH, but a positive correlation between the mononuclear cell counts and pCO2. However, no significant differences were observed between the nulliparous and multiparous groups in terms of the net weight of CB, total mononuclear cell counts and total CD34 cell counts.

Conclusions The findings of the present study show that the mononuclear cell counts are correlated with arterial CB pH and pCO2, suggesting the involvement of fetal hypoxia on the yield of mononuclear cells.

Statistics from Altmetric.com

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.