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Particle Counting Immunoassay for Urinary Cotinine. Comparison with Chromatography, Enzyme-linked Immunoassay and Fluorescence Polarization Immunoassay

  • Laurence M. Galanti , Juliette Dell'Omo , Danielle Vanbeckbergen , Pierre Dubois , Pierre L. Masson and César L. Cambiaso

Abstract

Urinary cotinine was measured according to its inhibitory activity on the agglutination of cotinine-coated latex particles by anti-cotinine antibodies, the agglutination being measured by optical counting of the remaining non-agglutinated particles (particle counting, PaC). The detection limit was 0.03 μg/ml and the practical range extended from 0.03 to 3.9 μg/ml. The correlation results of 320 urine samples with those of high pressure liquid chromatography, enzyme-linked (Coti-Tracq EIA, Serex Inc., Maywood, NJ, USA), and fluorescence polarization immunoassay (TDX instrument, Abbott, Abbott Park, IL, USA) were r = 0.90, r = 0.69, r = 0.87, respectively, whereas the correlation coefficients between the assays other than particle counting ranged from 0.62 to 0.88. PaC does not require any separation step and can thus be easily automated.

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Published Online: 2005-06-01
Published in Print: 1999-07-01

Copyright © 1999 by Walter de Gruyter GmbH & Co. KG

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