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Urinary polycyclic aromatic hydrocarbons as a biomarker of exposure to PAHs in air: A pilot study among pregnant women

Journal of Exposure Science & Environmental Epidemiology volume 22, pages 70–81 (2012)Cite this article

Abstract

Recent studies have linked increased polycyclic aromatic hydrocarbons (PAHs) in air and adverse fetal health outcomes. Urinary PAH metabolites are of interest for exposure assessment if they can predict PAHs in air. We investigated exposure to PAHs by collecting air and urine samples among pregnant women pre-selected as living in “high” (downtown and close to steel mills, n=9) and “low” (suburban, n=10) exposure areas. We analyzed first-morning urine voids from all 3 trimesters of pregnancy for urinary PAH metabolites and compared these to personal air PAH/PM2.5/NO2/NOX samples collected in the 3rd trimester. We also evaluated activities and home characteristics, geographic indicators and outdoor central site PM2.5/NO2/NOX (all trimesters). Personal air exposures to the lighter molecular weight (MW) PAHs were linked to indoor sources (candles and incense), whereas the heavier PAHs were related to outdoor sources. Geometric means of all personal air measurements were higher in the “high” exposure group. We suggest that centrally monitored heavier MW PAHs could be used to predict personal exposures for heavier PAHs only. Urine metabolites were only directly correlated with their parent air PAHs for phenanthrene (Pearson's r=0.31–0.45) and fluorene (r=0.37–0.58). Predictive models suggest that specific metabolites (3-hydroyxyfluorene and 3-hydroxyphenanthrene) may be related to their parent air PAH exposures. The metabolite 2-hydroxynaphthalene was linked to smoking and the metabolite 1-hydroxypyrene was linked to dietary exposures. For researchers interested in predicting exposure to airborne lighter MW PAHs using urinary PAH metabolites, we propose that hydroxyfluorene and hydroxyphenanthrene metabolites be considered.

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References

  • Aquilina N.J., Delgado-saborit J.M., Meddings C., Baker S., Harrison R.M., and Jacob P., et al. Environmental and biological monitoring of exposures to PAHs and ETS in the general population. Environ Int 2010: 36: 763–771.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Agency for Toxic Substances and Disease Registry (ATSDR). Toxicological profile for polycyclic aromatic hydrocarbons. US Department of Health and Human Services, Public Health Service; available from: http://www.atsdr.cdc.gov/ToxProfiles/tp69.pdf) 1995.

  • Bosetti C., Nieuwenhuijsen M.J., Gallus S., Carlo La Vecchia C., and Parazzini F. Ambient particulate matter and preterm birth or birth weight: a review of the literature. Arch of Toxicol 2010: 84: 447–460.

    Article  CAS  Google Scholar 

  • Brzeznicki S., Jakubowski M., and Czerski B. Elimination of 1-hydroxypyrene after human volunteer exposure to polycyclic aromatic hydrocarbons. Int Arch of Occ Environ Health 1997: 70: 257–260.

    Article  CAS  Google Scholar 

  • Buckley T.J., and Lioy P.J. An examination of the time course from human dietary exposure to polycyclic aromatic hydrocarbons to urinary elimination of 1-hydroxypyrene. Br J Ind Med 1992: 49: 113–124.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Canadian Environmental Protection Act (CEPA). Priority substances list assessment report — polycyclic aromatic hydrocarbons, 1994. Environment Canada, Health Canada; available from: http://www.hc-sc.gc.ca/ewh-semt/alt_formats/hecs-sesc/pdf/pubs/contaminants/psl1-lsp1/hydrocarb_aromat_polycycl/hydrocarbons-hydrocarbures-eng.pdf.

  • Castaño-Vinyals G., D’Errico A., Malats N., and Kogevinas M. Biomarkers of exposure to polycyclic aromatic hydrocarbons from environmental air pollution. J Occup Environ Med 2004: 61: e12.

    Article  Google Scholar 

  • Choi H., Perera F., Pac A., Wang L., Flak E., and Mroz E., et al. Estimating individual-level exposure to airborne polycyclic aromatic hydrocarbons throughout the gestational period based on personal, indoor, and outdoor monitoring. Environ Health Perspect 2008b: 116: 1509–1518.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Choi H., Rauh V., Garfinkel R., Tu Y., and Perera F.P. Prenatal exposure to airborne polycyclic aromatic hydrocarbons and risk of intrauterine growth restriction. Environ Health Perspect 2008a: 116: 658–665.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Delgado-Saborit J.M., Aquilina N., Meddings C., Baker S., Vardoulakis S., and Harrison R.M. Measurement of personal exposure to volatile organic compounds and particle associated PAH in three UK regions. Environ Sci Technol 2009: 43: 4582–4588.

    Article  CAS  Google Scholar 

  • Georgiadis P., Stoikidou M., Topinka J., Kaila S., Gioka M., and Katsouyanni K., et al. Personal exposures to PM 2.5 and polycyclic aromatic hydrocarbons and their relationship to environmental tobacco smoke at two locations in Greece. J Expo Anal Environ Epidemiol 2001: 11: 169–183.

    Article  CAS  PubMed  Google Scholar 

  • Hansen A.M., Mathiesen L., Pedersen M., and Knudsen L.E. Urinary 1-hydroxypyrene (1-HP) in environmental and occupational studies — a review. Int J Hyg Environ Health 2008: 211: 471–503.

    Article  CAS  PubMed  Google Scholar 

  • Jongeneelen F.J., van Leeuwen F.E., Oosterink S., Anzion R.B., van der Loop F., and Bos R.P., et al. Ambient and biological monitoring of cokeoven workers: determinants of the internal dose of polycyclic aromatic hydrocarbons. Br J Ind Med 1990: 47: 454–461.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Leroyer A., Jeandel F., Maitre A., Howsam M., Deplanque D., and Mazzuca M., et al. 1-Hydroxypyrene and 3-hydroxybenzo[a]pyrene as biomarkers of exposure to PAH in various environmental exposure situations. Sci Total Environ 2010: 408: 1166–1173.

    Article  CAS  PubMed  Google Scholar 

  • Li A., Schoonover T.M., Zou Q., Norlock F., Conroy L.M., and Scheff P.A., et al. Polycyclic aromatic hydrocarbons in residential air of ten Chicago area homes: concentrations and influencing factors. Atmos Environ 2005: 39: 3491–3501.

    Article  CAS  Google Scholar 

  • Li Z., Mulholland J.A., Romanoff L.C., Pittman E.N., Trinidad D.A., and Lewin M.D., et al. Assessment of non-occupational exposure to polycyclic aromatic hydrocarbons through personal air sampling and urinary biomonitoring. J Environ Monitor 2010: 12: 1110–1118.

    Article  CAS  Google Scholar 

  • Li Z., Romanoff L.C., Trinidad D.A., Hussain N., Jones R.S., and Porter E.N., et al. Measurement of urinary monohydroxy polycyclic aromatic hydrocarbons using automated liquid–liquid extraction and gas chromatography/isotope dilution high-resolution mass spectrometry. Anal Chem 2006: 78: 5744–5751.

    Article  CAS  PubMed  Google Scholar 

  • Li Z., Sandau C.D., Romanoff L.C., Caudill S.P., Sjodin A., and Needham L., et al. Concentration and profile of 22 urinary polycyclic aromatic hydrocarbon metabolites in the US population. Environ Res 2008: 107: 320–331.

    Article  CAS  PubMed  Google Scholar 

  • Llop S., Ballester F., Estarlich M., Ibarluzea J., Manrique A., Rebagliato M., Esplugues A., and Iñiguez C. Urinary 1-hydroxypyrene, air pollution exposure and associated life style factors in pregnant women. Sci Total Environ 2008: 407 (1): 97–104.

    Article  CAS  PubMed  Google Scholar 

  • Martí-cid R., Llobet J.M., Castell V., and Domingo J.L. Evolution of the dietary exposure to polycyclic aromatic hydrocarbons in Catalonia, Spain. Food Chem Toxicol 2008: 46: 3163–3171.

    Article  PubMed  Google Scholar 

  • Mucha A.P., Hryhorczuk D., Serdyuk A., Nakonechny J., Zvinchuk A., and Erdal S., et al. Urinary 1-hydroxypyrene as a biomarker of PAH exposure in 3-year-old Ukrainian children. Environ Health Perspect 2006: 114: 603–609.

    Article  CAS  PubMed  Google Scholar 

  • Perera F.P., Tang D., Whyatt R., Lederman S.A., and Jedrychowski W. DNA damage from polycyclic aromatic hydrocarbons measured by benzo [a] pyrene–DNA adducts in mothers and newborns from northern Manhattan, the world trade center area, Poland, and China. Cancer Epidemiol Biomarkers Prev 2005: 14: 709–714.

    Article  CAS  PubMed  Google Scholar 

  • Rajput N., Pyari A.A., Saini M.K., Kumari K.M., and Lakhani A. Assessment of PAH toxicity and mutagenicity in emissions from coal and biofuel combustion. J Environ Sci Eng 2010: 52 (3): 185–192.

    PubMed  Google Scholar 

  • Sahsuvaroglu T., Arain A., Kanaroglou P., Finkelstein N., Newbold B., and Jerrett M., et al. A land use regression model for predicting ambient concentrations of nitrogen dioxide in Hamilton, Ontario, Canada. J Air Waste Manage Assoc 2006: 56: 1059–1069.

    Article  CAS  Google Scholar 

  • Singer B., Hodgson A., and Nazaroff W.W. Gas-phase organics in environmental tobacco smoke: 2. Exposure-relevant emission factors and indirect exposures from habitual smoking. Atmos Environ 2003: 37: 5551–5561.

    Article  CAS  Google Scholar 

  • Suzuki Y, Niwa M, Yoshinaga J, Mizumoto Y, Serizawa S, and Shiraishi H Prenatal exposure to phthalate esters and PAHs and birth outcomes. Environ Int 2010: 36 (7): 699–704.

    Article  CAS  PubMed  Google Scholar 

  • Tonne C.C., Whyatt R.M., Camann D.E., Perera F.P., and Kinney P.L. Predictors of personal polycyclic aromatic hydrocarbon exposures among pregnant minority women in New York City. Environ Health Perspect 2004: 112: 754–759.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • US Environmental Protection Agency. Quality Assurance Guidance Document 2.12: Monitoring PM2.5 in Ambient Air Using Designated Reference or Class 1 Equivalent Methods, Human Exposure and Atmospheric Sciences Division. US EPA: Research Triangle Park, NC, 1998.

  • Viau C., Diakite A., Ruzgyte A., Tuchweber B., Blais C., and Bouchard M., et al. Is 1-hydroxypyrene a reliable bioindicator of measured dietary polycyclic aromatic hydrocarbon under normal conditions? J Chromatogr B 2002: 778: 165–177.

    Article  CAS  Google Scholar 

  • Viau C., Lafontaine M., and Payan J. Creatinine normalization in biological monitoring revisited: the case of 1-hydroxypyrene. Int Arch Occup Environ Health 2004: 77: 177–185.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We acknowledge the study participants and their families, Elaine Moore for recruiting and running the field study, and Keith Van Ryswyk and Hongyu You for assistance with the air sampling. This work was funded by Health Canada.

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Authors and Affiliations

  1. Air Health Science Division, Health Canada, 269 Laurier Avenue West, Ottawa, Ontario, Canada

    Elizabeth Nethery & Amanda J Wheeler

  2. Population Studies Division, Healthy Environments and Consumer Safety Branch, Health Canada, Tunney's Pasture, Ottawa, Ontario, Canada

    Mandy Fisher & Tye E Arbuckle

  3. Centers for Disease Control and Prevention, Atlanta, Georgia, USA

    Andreas Sjödin, Zheng Li & Lovisa C Romanoff

  4. Department of Obstetrics and Gynecology, McMaster University, Hamilton, Ontario, Canada

    Warren Foster

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  1. Elizabeth Nethery
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  2. Amanda J Wheeler
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  6. Lovisa C Romanoff
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Correspondence to Amanda J Wheeler.

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Nethery, E., Wheeler, A., Fisher, M. et al. Urinary polycyclic aromatic hydrocarbons as a biomarker of exposure to PAHs in air: A pilot study among pregnant women. J Expo Sci Environ Epidemiol 22, 70–81 (2012). https://doi.org/10.1038/jes.2011.32

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  • DOI: https://doi.org/10.1038/jes.2011.32

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